Flow Cytometry: OPSL Technology Meets Critical Needs
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چکیده
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Description of the technology Flow cytometry traditionally uses fluorochrome-labeled probes, such as antibodies, to identify cells expressing the targets of those probes. A sample stream carries single cells in suspension past a laser, exciting the fluorochromes, with quantitation of the emitted fluorescent signals from each cell via optical filters and photomultiplier tubes [1]. In mass cytome...
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The examination of the urine remains one of the most commonly performed tests in clinical laboratory practice. For over a century, standard microscopy analysis of urine has been considered as a golden standard. However, critical evaluation of sediment analysis has revealed a number of significant flaws, particularly in relation to centrifugation, the transfer error from the centrifugation tube ...
متن کاملFlow Cytometry
Flow cytometry is a technology that has impacted both basic cell biology and clinical medicine in a very significant manner. The essential principle of flow cytometry is that single particles suspended within a stream of liquid are interrogated individually in a very short time as they pass through a light source focused at a very small region. The optical signals generated are mostly spectral ...
متن کاملFlow Cytometry
Because it has been difficult to identify and separate malignant cells in human lymphoid malignancies, we have developed a flow cytometry-based fluorescent in situ hybridization (FISH) technique using immunoglobulin (Ig) heavy chain variable region (V H) gene probes. After obtaining the specific V H gene sequence expressed by the multiple myeloma IM-9 cell line and the malignant cells in five m...
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ژورنال
عنوان ژورنال: Optik & Photonik
سال: 2009
ISSN: 1863-1460
DOI: 10.1002/opph.201190058